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Information Name: | PowerQ ? qTaq DNA polymerase |
Published: | 2008-04-09 |
Validity: | 360 |
Specifications: | 200U/1000U |
Quantity: | 1.00 |
Price Description: | 60/250 |
Detailed Product Description: | Taq enzyme is mainly used for less demanding on the fidelity of PCR amplification and quantitative PCR, as a user to adjust the concentration of magnesium ions, all the buffer are not magnesium ions, magnesium ions separate packaging.
Taq enzyme characteristics
? cloning Thermu aquaticus DNA Polymerase Gene of Escherichia coli by the induction of the table, and then purified by three times over a separation of the recombinant protein of about 94 KD.
? A 5 'to 3' DNA synthesis capacity; no 3 'to 5' exonuclease activity.
? A 5 'to 3' exonuclease activity, especially suitable for quantitative PCR.
? A 3 'end of the function A plus the product can be used directly after purification TA cloning. ? No exogenous nuclease and bacterial DNA contamination. ? SDS - PAGE detection, the purity of "95%. ? Best extension temperature 72 ℃, the best Mg 2 + concentration, the concentration of the best dNTPS, extending speed 2000base/min. ? 10 × PCR buffer: 500mM KCl, 100mM Tris-HCl (pH, 25 ℃), 1% TritonX-100 and 15mM MgCl2. ? Storage buffer: 20mM Tris-HCl (pH), 100mM KCl, EDTA, 1mM DTT, 50% glycerol,% Nonidet-P40 and% Tween20. Taq enzyme unit defined Conditions in the 74 ℃ for 30 minutes catalyzed the incorporation of 10nmol dNTP into acid-insoluble material response to the amount of enzyme required for a unit. Reaction conditions: 50mM Tris-HCl (pH, 25 ℃), 50mM NaCl, 5mM MgCl2, 200mM dNTP (not marked and labeled with [3H] dTTP), 10mg of activated calf thymus DNA and, as a final reaction volume of 50ml. qTaq enzyme manual download (bulk user price negotiable, phone:) |
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